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How Can Our Lab Keep Track of Sub-Samples and Aliquots Once the Original Sample Is Split?

When a lab splits samples into aliquots, handwritten labels and lost links cause mix-ups. We build parent-child labelling and tracking at the bench.

Updated 2 min readBy SpiderHunts Technologies

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Quick answer — TL;DR

A single sample is often split into sub-samples for different sections, extracts, dilutions and retained portions, each labelled by hand with a shortened ID, and the link back to the original lives in a bench sheet. We build parent-child tracking that prints labels for every sub-sample from the registered sample, records where each goes, and lets anyone scan a tube to see what it is and where it came from.

A marker pen and a lot of trust

A soil sample arrives and is registered. In preparation, it is split: a portion for metals, a portion for organics, a portion kept as retained. Each portion is labelled with a marker pen, using the sample ID written a bit shorter because the tube is small. Metals digests it, producing an extract. Organics dilutes theirs. Each step creates new containers with more handwriting.

Almost always, it works. When it does not, a result is attributed to the wrong sample, and finding out how takes an investigation.

Why sub-samples get mixed up

  • Only the original sample has a printed label.
  • Sub-samples are labelled by hand with shortened IDs.
  • The link between parent and child is on a bench sheet.
  • Racks and positions are recorded inconsistently.
  • Nothing checks the right tube is loaded on the instrument.

Busy periods make it worse. When preparation runs behind, staff work faster, tubes sit in racks waiting for instruments, and the chance of two similar handwritten IDs being confused rises. The process relies on care at exactly the moment care is hardest to give.

What it costs

Mix-ups cause wrong results, amended reports and investigations. Staff spend time writing labels and checking them. Traceability from a result back to the original sample relies on paper.

StepHandwrittenParent-child tracking
SplitMarker pen labelsPrinted labels for each sub-sample
Link to parentBench sheetRecorded automatically
Extracts and dilutionsMore handwritingNew child labels with the chain recorded
Instrument loadingChecked by eyeScanned against the sequence
TraceabilityPaperResult to original sample in one view

The investigation after a suspected mix-up is often the biggest cost. Analysts pull bench sheets, check handwriting, compare instrument sequences and rerun retained portions. Even when it turns out there was no mix-up, the time is spent, and the report to the client may be delayed while the lab makes sure.

How we build it

  1. When a sample is split, the analyst chooses the sub-samples needed and labels print with unique barcodes, sized for the containers you use.
  2. Each label links to its parent, and each extract or dilution creates a further child with its preparation details.
  3. Rack positions are recorded by scanning.
  4. When loading an instrument, tubes are scanned against the sequence, and mismatches are flagged.
  5. Results for a child are linked back to the original sample in the LIMS.
  6. Anyone can scan a tube to see what it is, its parent and its history.

Labels are designed with your team so they are readable on the containers you use, including small vials and freezer tubes, and carry enough human-readable information for a quick visual check alongside the barcode.

What changes

No more marker pens for sample IDs. Links between samples and sub-samples are recorded automatically. Instrument sequences are checked by scan. Investigations can trace the chain from the record rather than from bench sheets.

Retained portions are linked to their parent too, so a retest request leads straight to the right tube. Investigations that used to need a day of bench sheet reading can often be answered from the tube history.

Is this your bench?

  • Sub-samples are labelled by hand.
  • Parent-child links are on bench sheets.
  • Mix-ups have led to amended reports.
  • Instrument sequences are checked by eye.
  • Tracing a result back to the original sample takes time.

FAQ

Frequently asked questions

The questions readers ask us after this guide.

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Ask about your project

Does our LIMS already do this?

Some do, partly. We check what yours supports and add what is missing.

What labels and printers work?

Standard thermal label printers and labels sized for tubes and vials, including freezer-resistant labels.

Does it slow analysts down?

Printing and scanning replace handwriting and checking, which usually takes less time.

What affects the cost?

The number of preparation steps and sections, printers and the LIMS connection.

Keep reading

More on Problems We Solve

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